Journal: Scientific Reports
Article Title: Conditional disruption of Nr5a1 directed by Sox9-Cre impairs adrenal development
doi: 10.1038/s41598-024-63264-9
Figure Lengend Snippet: Capsule formation during embryonic development. Representative images of double-label immunofluorescence for NR5A1 (green) and NR2F2 (red) was performed on parasagittal sections of embryos from control ( a – c ) and Sox9-Cre;Nr5a1 flox/flox ( d – f ) mice at E13.5, E14.5, and E15.5. Nuclei are stained blue with DAPI. AP, adrenal primordium; K, kidney; Li, liver. Scale bars, 100 µm in a , b , d , e, 200 µm in c , f .
Article Snippet: The primary antibodies were rabbit anti-NR5A1 , rabbit anti-SOX9 (ab185230; Abcam), rabbit anti-3βHSD (KO607; Trans Genic), rabbit anti-20αHSD/AKR1C1 (OAGA00409; Aviva Systems Biology), rabbit anti-Tyrosine Hydroxylase (AB152; Millipore), rabbit anti-Cleaved Caspase-3 (9661; Cell Signaling), goat anti-GATA4 antibody (sc1237; Santa Cruz Biotechnology), mouse anti- NR2F2/COUP-TFII antibody (PP-H7147; Perseus Proteomics), and mouse anti-BrdU antibody (B8434; Sigma-Aldrich).
Techniques: Immunofluorescence, Staining